Transfection of human HEK 293A and Hela cells with the BnRCH gene from Brassia napus

BnRCH gene was studied with heterologous expression system:it was stably transfected into human embryonic kidney cells 293A and human cervical carcinoma cells Hela.The stable transfectants were verified all right with RT-PCR,qPCR and Western blotting methods,which not only demonstrated that the plant gene BnRCH can express in animal cells normally,but also established the platform for studying BnRCH gene in animal cells.Meanwhile,the immunological cell chemistry method and green fluorescence protein(GFP) tracking method were used to study the sub-cellular localization of BnRCH protein in Hela cells and both methods indicated that BnRCH protein was evenly distributed inside Hela cells,which realized the preliminary investigation of the functions of BnRCH gene in Hela cells.