Energy-gathered pulsed ultrasonic-assisted hot water extraction of fish scale collagen

In this work,an energy-gathered pulsed ultrasonic-assisted procedure of fish scale collagen extraction was investigated.Response surface methodology( RSM) with a 3-factor,3-lever Box-Behnken Design( BBD) was conducted to ascertain the optimum ultrasound-assisted extraction parameters( extraction temperature X1,ratio of solvent to solid X2,ultrasonic time X3).The optimum conditions were determined to be extraction temperature 80℃,ratio of solid to solvent 40mL /g,extraction time 60min,pH6 and ultrasonic pulse mode 8s on and 5s off.Under this condition,the extraction efficiency of fish scale collagen could significantly increase from 67.31% to 81.25% and almost reached the model calculation value 81.63%.The FT-IR spectrum analysis revealed that ultrasonic-assisted treatment could cause the transfer of absorbance peak of acylamino residues I and II of fish scale collagen.