Optimization of Ultrasound-Assisted Extraction of Protein from Tea Residue by Response Surface Methodology

Ultrasound-assisted extraction of protein from tea residue was investigated in this manuscript.Effect of ultrasonic power,ultrasonic frequency,ultrasonic temperature,ultrasonic time,alkali concentration and material-liquid ratio on extraction yield of protein was investigated by single-factor experiment.Based on the results,the response surface methodology was used to optimize the extraction parameters and the optimum conditions listed as follows: e ultrasonic power 300 W,frequency 26 kHz,temperature 54 ℃,time 61 min,alkali concentration 0.35 mol/L and material-liquid ratio 1g∶27mL.Under these conditions,the protein extraction rate achieved at 86.5 %,increased by 37.2 % when compared with that of hot water alkali extraction.