Study on Callus and Tube Seedlings Culture of Leibnitzia anandria

[Objective] To study the culture conditions of Leibnitzia anandria callus and tube seedlings.[Method] With the flower stalks of Leibnitzia anandria as explants,the method of tissue culture was adopted to investigate callus induction and differentiation,differentiation and multiplication of adventitious buds,rooting and transplantation of tube seedlings.[Result] MS+0.5 mg/L 6-BA+2.5 mg/L 2,4-D was the best medium for induction of callus.The optimum medium for the differentiation of callus as well as the differentiation and multiplication of adventitious buds was MS+ AgNO3 0.8 mg/L+NAA 0.1 mg/L+6-BA 1.2 mg/L;the best rooting culture method of tube seedlings was inoculating the adventitious buds into 1/2MS+ NAA 0.1 mg/L after being treated with 1.2 mg/L IAA for 24 h;the transplanting survival rate was 92.8% and stable planting survival rate was 97.7%.[Conclusion] The tube seedlings survived retained all biological characteristics of the wild Leibnitzia anandria with exuberant growth,and could be used for culture.