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Determination of paeoniflorin in Paeoniae Radix Alba prepared by different processing methods by HPLC

Rui Zeng

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Abstract

Objective: To establish an HPLC method for determination of the contents of paeoniflorin in Paeoniae Radix Alba from Zhongjiang Sichuan and Bozhou Anhui. Method: The separation was performed on a Kromasil C 18 column (250 mm×4.6 mm, 5.0 μm) eluted with 0.1% phosphoric acid and acetonitrile (86:14) at a flow-rate of 1.0 mL·min -1 . The detection wavelength was set at 230 nm and the column temperature was set at 30 ℃. Result: The content of paeoniflorin in fresh Paeoniae Radix Alba was highest both in the medical material from Sichuan and Bozhou. Conclusion: The different processing methods have great influence on the content of the paeoniflorin. Therefore it is needed to combine the content determination and pharmacodynamics experiment in the standardation of the processing methods of Paeoniae Radix Alba.

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What this paper is about

Objective: To establish an HPLC method for determination of the contents of paeoniflorin in Paeoniae Radix Alba from Zhongjiang Sichuan and Bozhou Anhui. Method: The separation was performed on a Kromasil C 18 column (250 mm×4.6 mm, 5.0 μm) eluted with 0.1% phosphoric acid and acetonitrile (86:14) at a flow-rate of 1.0 mL·min -1 . The detection wavelength was set at 230 nm and the column temperature was set at 30 ℃. Result: The content of paeoniflorin in fresh Paeoniae Radix Alba was highest both in the medical material from Sichuan and Bozhou. Conclusion: The different processing methods have great influence on the content of the paeoniflorin. Therefore it is needed to combine the content determination and pharmacodynamics experiment in the standardation of the processing methods of Paeoniae Radix Alba.

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Available abstract

Objective: To establish an HPLC method for determination of the contents of paeoniflorin in Paeoniae Radix Alba from Zhongjiang Sichuan and Bozhou Anhui. Method: The separation was performed on a Kromasil C 18 column (250 mm×4.6 mm, 5.0 μm) eluted with 0.1% phosphoric acid and acetonitrile (86:14) at a flow-rate of 1.0 mL·min -1 . The detection wavelength was set at 230 nm and the column temperature was set at 30 ℃. Result: The content of paeoniflorin in fresh Paeoniae Radix Alba was highest both in the medical material from Sichuan and Bozhou. Conclusion: The different processing methods have great influence on the content of the paeoniflorin. Therefore it is needed to combine the content determination and pharmacodynamics experiment in the standardation of the processing methods of Paeoniae Radix Alba.

Key concepts: Paeoniflorin, Radix (gastropod), Chromatography, High-performance liquid chromatography, Phosphoric acid, Chemistry, Content determination, Traditional medicine

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