Study on Inhibition of Alpha-glucoside and Antioxidation of from Leaves and Twigs of Taxus chinensis var mairei in vitro

Objective:Inhibition of alpha-glucosidase and antioxidation of different extracts from the branches and leaves of Taxus chinensis var mairei were studied in vitro to supply the separating base of the effective chemical components.Method:The inhibitory activities of α-glucoside were assayed in external.The antioxidant activities of these extracts were assessed bytwo complementary test systems,namely 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity and ferric reducing antioxidant power(FRAP) assay.Result:The ethyl acetate extract showed strong activity in the inhibitory activities of α-glucoside.(IC50 27.854 1 mg · L-1) The ethanol extract(IC50 29.215 5 mg · L-1)and n-butanol extract(IC50 38.913 0 mg · L-1) displayed lower activity.Petroleum ether fraction(IC50 128.729 5 mg · L-1) and water fraction(IC50 74.505 9 mg · L-1) have a little activity.The antioxidant activities:ethanol extract(DPPH IC50 6.370 7 mg · L-1,FRAP 4.266 mmol · g-1)ethyl acetate extract(DPPH IC50 9.535 8 mg · L-1,FRAP 3.275 mmol · g-1)n-butanol extract(DPPH IC50 20.461 3 mg · L-1,FRAP 1.498 mmol · g-1)water fraction(DPPH IC50 26.685 5 mg · L-1,FRAP 0.678 mmol · g-1)petroleum ether fraction.Conclusion: The components of inhibition α-glucoside could be testified in the ethyl acetate extract and n-butanol extract.Antioxidative components were in the ethyl acetate extract.