α-Glucosidase Inhibitory and Antioxidant Activity of Ardisia crenata

To investigate the α-glucosidase inhibitory and antioxidant activity of Ardisia crenata,96-microplate-based method was used to assay α-glucosidase inhibitory activity of A.crenata and antioxidant activity was determined by the method of DPPH,ABTS,and FRAP.The results showed that the ethyl acetate extract(IC50=39.27 μg/mL) had the highest α-glucosidase inhibitory activity,the petroleum ether extract came second(IC50=56.11 μg/mL),and the n-butanol extract was the weakest(IC50=62.05 μg/mL).But all of them showed higher activity than that of Acarbose(IC50=1081.27 μg/mL).The ethyl acetate extract showed the highest antioxidant activity which was higher than that of n-butanol extract.The DPPH radical scavenging activity of ethyl acetate extract(IC50=38.55 mg/L) was half of BHT(IC50=18.71 mg/L) while ABTS radical scavenging activity(IC50=3.60 mg/L) was higher than that of BHT(IC50=7.44 mg/L) and lower than that of BHA(IC50=1.74 mg/L).It exhibited the ferric reducing antioxidant power(FRAP=512.99 ±6.80 μmol TE/g) which was almost one third of BHT(FRAP=1581.68±97.41 μmol TE/g).The results indicated that the ethyl acetate extract of A.crenata exhibited the strongest activity of α-glucosidase inhibitory and antioxidant activities.