Influence of "Qiyeling Granule" on apoptosis of Lewis lung cancer and MMP9 expression in C57 mice

Objective To observe the effects of Qiyeling in suppressing tumor growth,and infiltration and metastasis of tumor cells of C57 mice with transplanted tumor.Methods C57 mice with transplanted tumor were randomized into four groups: model group(group A),chemotherapy group(group B),Qiyeling Granule Group group(group C) and chemotherapy plus Qiyeling Granule Group group(group D).The living quality and suppression of tumor growth of C57 mice were observed;the in situ cell apoptosis analysis kit was used for count of apoptosis,and hybridisation was used to test MMP9 expression.Results ①Quality of life was better in groups C and D than in groups A and B.②Suppression rate of tumor growth was 74.47% in group D,obviously higher than that in group A(P0.01),and the average tumor weight was lower in group B,C and D than in group A(all P0.05).③In situ cell apoptosis analysis showed that tumor apoptosis was higher in group B,C and D than in group A(P all0.05),higher in group D than in group C(all P0.05).④In situ hybridisation test indicated that MMP9 expressions were significantly lower in group B,C and D than in group A(all P0.05).Conclusions Qiyeling can improve the living quality of C57 mice,and suppress the growth of Lewis lung cancer cell by inducing apoptosis of cancer cells and inhibiting MMP9 expression.