Effects of "Feiyanning Granule" on MDM2-P53 Bio-axis of Lewis Lung Cancer Cell Cycle G1/S Checkpoint Signal Pathway

Objective:To observe the effects of Feiyanning Granule on MDM2-P53 bio-axis of Lewis lung cancer cell cycle G1/S checkpoint signal pathway.Methods:C57BL/6 mice were applied,and the Lewis lung cancer model mice were established.The model mice were randomly divided into six groups:model group(A),cisplatin group(B),Feiyanning decoction group(C),Feiyanning Granule low-,medium-and high-dose groups(D,E,F),with corresponding drug administered.The tumour inhibiting rate,tumour weight,body weight and body weight without tumour were observed.Cell cycle distribution was detected by flow cytometry(FCM),and the expressions of MDM2 and P53 mRNA in tumour tissues were tested by RT-PCR.Results:The tumour weight in group E was lower than that in group A(P0.01),and had no statistical difference compared with group B.The body weight of group E was significantly higher than that of groups A and B(P0.05,P0.01);the body weight without tumour of group E was obviously higher than that of groups A,B and C(P0.01,P0.05).The FCM analysis showed that the proportion of G0/G1 phase cell cycle in group E was increased,and the cancer cell proliferation index(PI) was decreased compared with groups A and B(P0.01).The RT-PCR results showed that the level of MDM2 mRNA expression of group E was lower than that of groups A,B and C(P0.01),and the level of P53 mRNA expression of group E was higher than that of groups A,B and C(P0.01).There were no mice alive in group F,and the death had relationship with granule solubility.Conclusion:Regulating the MDM2-P53 bio-axis of cell cycle G1/S checkpoint signal pathway is one of the important pathways for Feiyanning Granule to inhibit lung cancer cell proliferation in Lewis lung cancer mice.