Study on Universal Culture Medium for Inducing Callus of Indica Rice

In order to find a general inducing medium for cultivars of Indica rice,an orthogonal text containing three factor,which were the basic medium(NB,N6,CC and MB),2,4-D(2.0,3.0,4.0 and 5.0 mg/L)and 6-BA(0,0.2,0.4 and 0.5 mg/L) was designed.There were 16 medium combinations based on same nutrient components(300 mg/L casein hydrolysate + 500 mg/L proline + 250 mg/L glutamine + 30g /L sucrose + 8g/L agar) with three cultivars of Indica rice,which were Minghui63,IR64,and RD23,were employed for inoculation on the 16 medium combinations and culture in the same conditions.The callus induction rate and quality of each combination were evaluated.The results indicated that the 2,4-D significantly increased callus inducing rate,the 6-BA significantly decreased the quality of callus and the MB medium was the most suitable basic medium for callus inducing.The general medium for callus inducing for cultivars of Indica rice was the combination including MB medium,5.0 mg/L 2,4-D.The culture condition for induction was in the dark for 20 days with 28 ℃.The confirmed experiment showed that the medium was suitable for most cultivars of Indica rice.