Transformation,Inducing and Regeneration of Callus Initiated from Mature Embryos of Indica

In this study we established an efficient indica regeneration system initiated from mature embryos of Minghui 86,Qishanzhan and Zhongyouzao8.The results showed that MS medium was the most suitable for callus induction and differentiation.4% maltose could produce the highest rate of callus induction and differentiation.Adding 2 mg/L 2,4-D,0.5 mg/L KT,1 mg/L IAA,0.5 mg/L 6-BA,2.878 g/L L-proline,1 g/L casein hydrolysate in the callus induction medium,it were continuous lighted for 7 days at 32℃,2 mg/L KT,1 mg/L IAA,2 mg/L 6-BA,1 g/L casein hydrolysate supplemented in differentiation medium could significantly improve the callus induction rate and regeneration rate.It was also under continuous light at 32℃.The average induction rate was 84.9% and regeneration rate was 80.4%.By the Agrobacterium-mediated transformation method,hyg-resistant calli were obtained,using indica Minghui 86 as a genetic transformation receptor.GUS staining with resistant calli showed that the reporter gene had been integrated into the rice chromosome and had expressed.This transformation method based on the above-mentioned regeneration system increased the transient expression efficiency to 55.8%.