Effects of at microwave various temperatures on the morphology and structure of canine bile duct

Objective To observe the pathological morphological changes of canine bile duct caused by the action of different temperature microwave and to study the safe dose for the application of microwave to treatment of bile duct carcinoma. Methods Single meedle microwave antena was inserted into canine bile duct by surgical operation to apply hyperthermia to the bile duct cavity with different temperatures. The canines were killed and the samples were collectedat different times after the operation. The changes of bile duct tissue were observedwith light and electron microscopies. Results White blood cells were found to infiltrate into the tissue of the bile duct under microwave at 43℃ for 30 minutes. The mucosal epithelial cells of the bile duct were found degen-eratrd and necrostic at 45℃ for 30 minutes. The pathological injuries were severe. Conclusions Heating of 43℃ for 30 minutes may be the safer dose to treat bile duct carcinoma with microwave.