The establishment of rabbit model for bile duct electric burn

Objective:To establish a safe and reliable rabbit model of bile duct electric burn. Methods:Thirty rabbits were divided into 5 groups equally.The common bile duct was isolated during laparotomy under anesthesia.The high-frequency operation unit,self-made time-controlling device and modified electric knife were used in this study,the common bile duct of rabbits in each group was coagulated for 1s with 10W,15W,20W,25W,30W respectively,and then the wall thickness of residual bile duct was measured with the pathological observation. Results:Coagulation with no more than 25W did not cause immediate bile leakage,but 30W of power,without exception,caused immediate bile leakage,and pathology showed wall defect.There was a significant difference in the mean thickness of residual bile duct wall in each group(P0.01) and the thickness was negatively correlated with power(r=-0.887). Conclusions:This high-frequency operation unit with 20W power and 1s coagulation can make a safe,ideal model of bile duct electric burns,which can be used for further researches.