MMP-7 gene expression in gastric cancer and its significance

Purpose:To study the expression of MMP 7 gene in gastric cancer tissue and different stomach cancer cell line and explore its clinical significance.Methods:Reverse transcription polymerase chain reaction (RT PCR) was used to detect the expression of MMP 7 mRNA in 35 gastric cancer samples and the surrounding normal tissues and stomach cancer cell line. The ability of the invasion was observed by Boyden Chamber invasion assay. Results:The expression rate of MMP 7 mRNA in 35 gastric cancer samples and the surrounding normal tissues was 60% and 11%, respectively. The expression level of MMP 7 gene in gastric cancer was much higher than that in the surrounding normal tissues[(0 627±0 276)vs(0 237±0 12), P 0 05] Furthermore, the extremely high expression level of MMP 7 mRNA was detected in tumors with metastasis. The expression level of MMP 7 gene was strongly positive in low differential cell line .It was weakly positive in highly differentiated cell line. The number of cells migrating through the membrane obviously decreased after PS asODN treatment (15.6(1.21 )compared with the control group and PS sODN and PS mODN (75.4±6.16,53.8±7.32,58.4±5.87 respectively)( P 0.05).Conclusions:The expression level of MMP 7 mRNA is high in stomach cancer , especially in tumors with metastasis. It is strongly positive in lowly differentiated cell line, and weakly positive in highly differentiated cell line. MMP 7asODN reduced significantly the invasion ability of the cancer cell. MMP 7 play a role in tumor invasion and metastasis. [