Tea polyphenols induced apoptosis and increased the synergic antitumor effect of THP in the EJ line

Objective To study the molecular mechanism of cell proliferation inhibited by tea polyphenols in bladder carcinoma cell line EJ.Methods Light microscopy,MTT assay,flow cytometry,laser confocal scanning microscopy were applied to investigate the effects of tea polyphenols on cellular mitochondrial function,concentration of THP and cytoplasmic Ca 2+ and related molecular mechanisms.Results After treatment of EJ cells with tea polyphenols,the proliferating cells was obviously changed as morphologic analysis,MTT assay,and the survival rate of cells decreased from 100.0% to 25.4%. increased tea polyphenols concentrations,the number of cells in S phase was obviously decreased from 9.2 to 5.1, following the number of cells in phase G1 were elevated from 50.9 to 62.7. It suggested that tea polyphenols could arrest the cell cycle at G0/G1 checkpoint.The Ca 2+ concentrations were obviously raised 10.1 fold.teamented the cells with tea polyphenols and THP,which could arrest the cell cycle at G0/G1 checkpoint.The Ca 2+ concentrations were also obviously raised beyond 48 hour.statistically significant correlation ( P 0.005) were observed between tea polyphenols and THP.Conclusion Tea polyphonls could arrested the cell cycle and inhibited cells proliferation of bladder carcinoma,up regulated the Ca 2+ and THP concentrations in EJ line。Mitochondria function might also be involved in these events.