2008•Acta Medicinae SinicaRequires access

Study of the immobilization of papain on nano-silica

Xian‐Li Ma

Abstract

Objective: To study the properties of immobilized papain.Methods: Papain was immobilized on nano-silica using glutaraldehyde as the cross-linker.The optimum immobilization conditions of papain on the nano-silica,enzyme properties and kinetics parameters were studied.Results: The optimum temperature and pH of immobilized enzyme was 75℃ and 8.0,respectively,and the enzyme activity could reach up to 61.5 %.Conclusion: Immobilization has no obvious influence on enzyme activity compare with dissociate papain.

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Key concepts: Papain, Glutaraldehyde, Chemistry, Immobilized enzyme, Nano-, Kinetics, Enzyme, Chromatography