Cloning of bovine myf6 gene and expression of gene in fibroblasts

myf6 gene was a member of the MRFs family.It controlled the cell proliferation and differentiation.In this article,genes of the myf6 were cloned and eukaryotic expression vector pIRES2-EGFP-myf6 was constructed.Liposome techinigue was used to transfect Luxi cattle fibroblasts,and by G418 selection,a stable transfected cell line was gained.The effects of fibroblasts were analyzed by Western blot and Real-time PCR.Compared with the control group,the results showed that after the fibroblasts transfected by plasmid,protein and mRNA expression levels of myf6 both increased(P0.01),mRNA expression of the muscle creatine kinase genes increased(P0.05),and mRNA expression of myosin light chain gene had increased(P0.01).Morphology observations indicated that fibroblasts did not fuse to myotubes.The results suggested that myf6 gene was expressed in fibroblasts and promoted fibroblasts differentiation.