Effects of human insulin-like growth factor 1 gene transfection on proliferation of NIH3T3 fibroblasts

Objective To study the effects of human insulin-like growth factor 1(hIGF-1) gene transfection on the proliferation of NIH3T3 fibroblasts.Methods The plasmid of pcDNA3.1-hIGF-1 was transfected into NIH3T3 fibroblasts by using Lipofectin method.The positive cell clones were selected with G418 and cultured for 4 weeks.The stable expression of hIGF-1 in the positive cells was determined by in situ hybridization and immunocytochemical analysis.MTT assay and flow cytometer analysis were used to observe the proliferation of NIH3T3 fibroblasts.Results hIGF-1 mRNA and protein expressed in NIH3T3 fibroblasts transfected with pcDNA3.1-hIGF-1 by in situ hybridization and immunocytochemical analysis.MTT assay showed the A value of transfected NIH3T3 fibroblasts rose,compared with untransfected NIH3T3 fibroblasts group,the difference was significant(P0.01).Cellular proportion in S period(59.3%) was increased and it in G_1 periods was decreased(27.2%) after transfection by flow cytometer measurement.Conclusion The stable expression of hIGF-1 in NIH3T3 fibroblasts transfected with pcDNA3.1-hIGF-1 is obtained.Gene transfection of hIGF-1 can stimulate the proliferation of NIH3T3 fibroblasts.