Mapping of Rice Fertility Restorer Genes for Cytoplasmic Male Sterility Detected in a Restorer Line ZSP-1
Cun Yang
Abstract
Cun Yang
Abstract
The pollen fertility of F 2 individuals of crosses Zhenshan 97A/ZSP 1 and Xing A/ZSP 1 was investigated. The segregation ratio of fertile to sterile plants was fit to 15∶1. The results show that fertility restoration of the cytoplasmic male sterility in these crosses was controlled by two independent restorer genes. The RFLP markers, that are linked closely to the known restorer genes Rf 3 and Rf 4 for WA CMS, were used to map the restorer genes found in the restorer line ZSP 1. The results of linkage analysis indicated that the restorer genes in ZSP 1 were located in the same regions of Rf 3 and Rf 4 .
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The pollen fertility of F 2 individuals of crosses Zhenshan 97A/ZSP 1 and Xing A/ZSP 1 was investigated. The segregation ratio of fertile to sterile plants was fit to 15∶1. The results show that fertility restoration of the cytoplasmic male sterility in these crosses was controlled by two independent restorer genes. The RFLP markers, that are linked closely to the known restorer genes Rf 3 and Rf 4 for WA CMS, were used to map the restorer genes found in the restorer line ZSP 1. The results of linkage analysis indicated that the restorer genes in ZSP 1 were located in the same regions of Rf 3 and Rf 4 .
Key concepts: Sterility, Cytoplasmic male sterility, Biology, Fertility, Gene, Pollen, Restriction fragment length polymorphism, Genetic linkage