2008•Zhonghua zhongyiyao xuekanRequires access
Empirical Study on Effect of Different Extracts of Rhizoma Amorphophalli on the Proliferation of Cell SGC-7901
Lei Pan
Open publisher page 1 citations
Abstract
Objective:To observe the effect of different extracts of Rhizoma Amorphophalli on the proliferation of cell SGC-7901,and explore the possibility mechanism of them.Methods:Different extracts of Rhizoma Amorphophalli were extracted by the technique of solvent-refining; effect of different dose groups of extracts on cell SGC-7901 were tested by MTT and growth curve method; effects of the extracts of petroleum ether on cell cycle and apoptosis were inspected by flow cytometry.Results:The extracts of ethanol, petroleum ether and ethyl acetate of Rhizoma Amorphophalli have inhibitory effect on the proliferation of cell SGC-7901,and the extract of petroleum ether has much more evident effect, showed a dose-effect curve. Growth curve method showed the extract of petroleum ether and ethyl acetate of Rhizoma Amorphophalli had a time-effect effect on the proliferation of cell SGC-7901. Flow cytometry detection showed the extract of petroleum ether of Rhizoma Amorphophalli can block the cell cycle of SGC-7901 at G0/G1 period,and the mechanism possibly related to inducing apoptosis of tumor cell.Conclusion:The extract of petroleum ether of Rhizoma Amorphophalli is possibly one of antineoplastic parts of the plant.