Experimental Study on Effect of Petroeum Ether Extract of Rhizoma Amorphophalli on Apoptosis of Sgc-7901

Objective:To study the underlying principles of cell apoptosis which caused by Rhizoma amorphophalli with human gastric cancer cells SGC-7901.Method:Test the effect caused by petroleum ether extract of Rhizoma amorphophalli on gastric cancer SGC-7901 cells proliferation with MTT.Take different concentrations of petroleum ether extract of Rhizoma amorphophalli acting on SGC-7901 cells.Analyze the expressions of Survivin,Bcl-2,Bax and Cleaved-Caspase-9 with Western Blot method.Results:Different concentrations of petroleum ether extract of Rhizoma amorphophalli had diffierent inhibiting effect on SGC-7901 cells proliferation.With the increase of concentration,the inhibition gradually enhanced.After 24 hours' acting on the SGC-7901 cells,all of different concentrations of petroleum ether extract of Rhizoma amorphophalli can obviously down-regulate the expressions of Survivin protein and Bc1-2 protein.At the same time it can obviously up-regulated the expressions of Bax protein and Cleaved-Caspase-9 protein.Conclusion:The possible mechanism that make SGC-7901 cells apoptosis of petroleum ether extract of Rhizoma amorphophalli may be related to the influence on the expressions of above proteins.