Effects of Aspongopus chinensis Dallas extracts on cell proliferation and cell cycle of SGC-7901 and HepG_2 cell lines

AIM To study the effects of crude extracts of Aspongopus chinensis Dallas on cell proliferation and cell cycle of SGC-7901 and HepG2 cell lines.METHODS The crude extracts were extracted by chloroform infusion.The antiproliferative effects of crude extracts were assessed by MTT assays.The cell cycle was measured by flow cytometry.RESULTS The crude extracts could inhibit the proliferation of SGC-7901 and HepG2 cell lines in a dose-dependent manner.SGC-7901 and HepG2 cell lines were sensitive to crude extracts with an IC50 of 1 193.52 and 964.34 μg/mL,respectively.From the result of flow cytometry analysis,crude extracts,as compared with the untreated cells,caused significant reduction in cell ratio at the S and G2/M phases,and an increase in cell ratio at the G0/G1 phases.CONCLUSION The crude extracts could inhibit proliferation of both cancer cell in vitro,and especially arrest human liver cancer cells at the G0/G1 phase of the cell cycle for HepG2.