Expression of Skp2 and its Correlation with p27 in Laryngeal Squamous Cell Carcinomas

Objective To detect protein expression of skp2 and p27 in laryngeal squamous cell carcinomas(LSCC).Correlation between tumor expression of Skp2 and p27 and clinicopathological characteristics of patients with LSCC was studied.Methods the expression of Skp2 and p27 in them were examined by immunohistochemically using highly specific antibodies on formalin-fixed,paraffin-embedded tissue section from 58 LSCC,12 of laryngeal precancerous lesion(LPL) and 26 normal laryngeal tissues(NLT).Results We observed the more significantly increased expression of Skp2 in LSCC and LPL groups than those in NLT group.Overexpression of Skp2 in LSCC strongly correlated with the TNM stage,lymph node metastasis,loss of tumor differentiation and loss of p27.A significant decrease in overall survival was observed in patients expressed high Skp2 levels.Conclusion These results suggest that Skp2 are involved in p27 down-regulation and may have an important role in the development of aggressive behavior in LSCC.Overexpressing of Skp2 may be used as prognostic markers for overall survival in LSCC.