ScholarLens Back to search
2005•Chinese Journal of Current Advances in General SurgeryRequires access

Antisense oligonucleotide mediated inhibition on telomerase activity in gallbladder carcinoma cell

Bin Jin

Open publisher page 0 citations

Abstract

Objective:To study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC SD cell.Methods:We design the antisense,sense,and random oligonucleotide with phosphoric acid modification for the hTR(Human Telomerase RNA)template sequence.MTT and PCR methods were used to observe the inhibition on telomerase activity and cell proliferation of GBC SD cell ,and fibroblast cells were used as control group.Results:PS ODN can lead to the reduction of cell survival rate of GBC SD cell,wich dosage dependence.Tne experimental group cell detected by scanning electron appeared apoptotic feature.Conclusion:PS ODN can inhibit telomerase activity of GBC SD cell effectively and induce the cell apoptosis.

About this research paper

What this paper is about

Objective:To study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC SD cell.Methods:We design the antisense,sense,and random oligonucleotide with phosphoric acid modification for the hTR(Human Telomerase RNA)template sequence.MTT and PCR methods were used to observe the inhibition on telomerase activity and cell proliferation of GBC SD cell ,and fibroblast cells were used as control group.Results:PS ODN can lead to the reduction of cell survival rate of GBC SD cell,wich dosage dependence.Tne experimental group cell detected by scanning electron appeared apoptotic feature.Conclusion:PS ODN can inhibit telomerase activity of GBC SD cell effectively and induce the cell apoptosis.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC SD cell.Methods:We design the antisense,sense,and random oligonucleotide with phosphoric acid modification for the hTR(Human Telomerase RNA)template sequence.MTT and PCR methods were used to observe the inhibition on telomerase activity and cell proliferation of GBC SD cell ,and fibroblast cells were used as control group.Results:PS ODN can lead to the reduction of cell survival rate of GBC SD cell,wich dosage dependence.Tne experimental group cell detected by scanning electron appeared apoptotic feature.Conclusion:PS ODN can inhibit telomerase activity of GBC SD cell effectively and induce the cell apoptosis.

Key concepts: Telomerase, Oligonucleotide, Cell growth, Sense (electronics), Cell, Molecular biology, Telomerase reverse transcriptase, Apoptosis

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Antisense oligonucleotide mediated inhibition on telomerase activity in gallbladder carcinoma cell — Research Paper | ScholarLens