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2002•Carcinogenesis,Teratogenesis and MutagenesisRequires access

EFFECT OF ANTISENSE OLIGONUCLEOTIDE AGAINST hTR RNA ON THE TELOMERASE ACTIVITY AND APOPTOSIS IN K562 CELLS

Jie Hu, Yang Shengli, Jiang Dong-xia

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Abstract

Purpose: To study the inhibitory effect of human telomerase activity and the apoptosis by an antisense oligonucleotide against human telomerase RNA(hTR) in K562 cells. Methods: Telomerase activity of K562 cells with antisense treatment was measured using the TRAP assay, and apoptosis was studied by means of TUNEL method. Results: The telomerase activity was effectively inhibited, and the apoptosis was induced by the antisense treatment. Conclusion: Antisense oligonucleotide against hTR can effectively inhibit the telo \{merase\} activity of K562 cells.

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What this paper is about

Purpose: To study the inhibitory effect of human telomerase activity and the apoptosis by an antisense oligonucleotide against human telomerase RNA(hTR) in K562 cells. Methods: Telomerase activity of K562 cells with antisense treatment was measured using the TRAP assay, and apoptosis was studied by means of TUNEL method. Results: The telomerase activity was effectively inhibited, and the apoptosis was induced by the antisense treatment. Conclusion: Antisense oligonucleotide against hTR can effectively inhibit the telo \{merase\} activity of K562 cells.

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Available abstract

Purpose: To study the inhibitory effect of human telomerase activity and the apoptosis by an antisense oligonucleotide against human telomerase RNA(hTR) in K562 cells. Methods: Telomerase activity of K562 cells with antisense treatment was measured using the TRAP assay, and apoptosis was studied by means of TUNEL method. Results: The telomerase activity was effectively inhibited, and the apoptosis was induced by the antisense treatment. Conclusion: Antisense oligonucleotide against hTR can effectively inhibit the telo \{merase\} activity of K562 cells.

Key concepts: Telomerase, K562 cells, Apoptosis, Oligonucleotide, TUNEL assay, Molecular biology, Sense (electronics), Telomerase reverse transcriptase

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EFFECT OF ANTISENSE OLIGONUCLEOTIDE AGAINST hTR RNA ON THE TELOMERASE ACTIVITY AND APOPTOSIS IN K562 CELLS — Research Paper | ScholarLens