Effect of antisense RNA for hTR on telomerase activity and cell apoptosis in human gallbladder cancer

Objective To explore the effect of antisense telomerase RNA on telomerase activity and cell apoptosis in human gallbladder cancer.Methods According to the hTR cDNA sequence and multiclone site's physical map of eukaryon express vector,the antisense RNA and sense RNA sequence were synthesized in vitro and transduced into the eukaryon expression vector pTriEx 4 separately.By using the method of Lipofectamine transfection,the carcinoma of gallbladder was transfected.Telomerase activity of GBC SD cells was examined by telomeric repeat amplification protocol (TRAP).Apoptosis was detected by morphology and flow cytometry.Results Antisense RNA could inhibit telomerase activity and cell proliferation of GBC SD cell.After antisense RNA transfection,the cell ratio of G 0/G 1 phase was increased obviously,and the cell ratio of S phase decreased obviously.The apoptosis rate was 11.10% and 29.02% on the day 10 and 13 after antisense RNA transfection.Conclusion Antisense RNA can inhibit telomerase activity of GBC SD cell effectively and induce apoptosis.