Primary culture and identification of ICR mice sertoli cells

Objective To establish culture method of ICR mice sertoli cells and to study the effects of the environmental chemicals on the male reproductive system at the cell molecular level.Methods The Sertoli cells were derived from the ten days old mice testes by twice collagenase digestion,then they were treated with Tris-HCl to remove spermatogenic cells.To Identify the Sertoli cells with Feulgen staining,and to view the morph of cultured Sertoli cells under the microscope.Results Sertoli cells grew vigorously,the purity of the cells were over 95 percent,and bipolar Corpuscula in nucleus was clearly observed after Feulgen staining.Conclusion Twice collagenase digestion to separate Sertoli cells and Feulgen staining to identify is simple and feasible and the purity is high.The results provided a theoretical foundation for study on the male reproductive toxicity of the environmental chemicals in vitro.