Primary Culture and Identification of Testis Sertoli Cells in Calf

To search for a simple and economical method to separate and purify calf testis sertoli cells and to identify the sertoli cells with different method,the method of collagenase and typin was sequentially used to separate sertoli cells.After 5 hours the culture medium was replenished and after 24 hours the treatment with Tris-HC was done to remove spermatogenic cells.The sertoli cells were identified with Feulgen staining.The expression of FAS-L was examined by SABC staining.The result indicated that the purity of sertoli cells accounted for over 90% of all cells harvested.Bipolar corpuscula in nucleus was clearly observed after Feulgen staining.Sertoli cells expressed FAS-L.The conclusion was the method of isolation and culture of sertoli cells from calf was established.Feulgen staining and SABC staining were useful method for identification of the sertoli cells.