THE PRIMARY CULTURE AND IDENTIFICATION OF RAT SERTOLI CELLS

Objective We used modified method to separate and culture Sertoli cells in order to set up a stable primary culture system of Sertoli cells.Besides,a simple but feasible way was founded to identify Sertoli cells. Methods We used enzymatic digestion to separate Sertoli cells,and treated with Tris-HCl to remove spermatogenic cells.Identified the Sertoli cells with Feulgen staining,then viewed the morphology of cultured Sertoli cells under the microscope. Results The purity of Sertoli cells came to 95 percent.the total number of Sertoli cells we got from one testicle was up to 10~7,bipolar corpuscula in nucleus was clearly observed after Feulgen staining.Conclusion The method of enzymatic digestion and Feulgen staining to separate and identify Sertoli cells is simple and feasible with high purity.