Changes of Distribution and Antimicrobial Resistance of Gram-negative Bacilli in Intensive Care Unit

Objective To find out dynamic changes of distribution and antimicrobial resistance of gram-negative bacilli from intensive care unit(ICU) of our hospital.Methods Isolated bacteria were identified by API system,and its antimicrobial resistance was determined by Kirby-Bauer method.Data of isolation rate and drug resistant rate were analyzed by WHONET 5.4 software.Results Total 711 gram-negative bacilli isolates collected during three years.The most common species were Acinetobacter baumannii(27.3%),Pseudomonas aeruginosa(PA)(27.1%),Escherichia coli(11.4%),Klebsiella pneumonia(7.7%) and Stenotrophomonas maltophilla(4.6%).The multi-drug resistant strains of Acinetobacter baumannii exhibited the increasing trend year by year.While the resistant rate of PA has been decreased.In 2007,2008 and 2009,the detection rates of the ESBLs-producing Escherichia coli were 52.6%,63.2% and 71.4%,respectively,those of Klebsiella pneumonia were 50.0%,53.1% and 35.7%,respectively.Conclusion Gram-negative bacilli were main pathogen in ICU and non-fermentative bacilli were predominant pathogen.Monitoring the changes of distribution and drug resistance were very important to make the antibiotics effective.