Distribution and drug resistance of common gram-negative bacilli in an intensive care unit

Objective To investigate the distribution and drug resistance of common gram-negative bacilli in intensive care unit(ICU) in a hospital.Methods The species and drug susceptibility test of the common gram-negative bacilli isolated from ICU between 2008 and 2010 were analyzed retrospectively.Bacteria were identified and performed antimicrobial susceptibility test with VITEK-2(bio-Merieux) system.The date were analyzed by the software WHONET-5.5.Results Of 1 551 gram-negative bacilli isolates,68.06%were isolated from sputum;among the common gram-negative bacilli,the first one was Acinetobacter baumannii,which accounting for 25.40% (394 strains),followed by Klebsiella pneumoniae(21.66%,336 strains),Escherchia coli(16.76%,260 strains), and Pseudomonas aeruginosa(15.86%,246 strains),etc;198(58.93%) strains of Klebsiella pneumoniae and 174 (66.92%) of Escherichia coli were extended-spectrum(Mactamase-producing strains.The common gram-negative bacilli had very low resistant rates to piperacillin/tazobactam,imipenem,cefoperizone/sulbactam and amikacin,the resistant rates was 19.23%-31.73%,9.23%-37.40%,14.88%-32.23%,and 22.92%-27.66%,respectively. Conclusion Multi-drug resistance among the common gram-negative bacilli in ICU is very serious.In order to reduce the emergence of drug-resistant bacterial strain,it is important to use antimicrobial agents correctly based on drug susceptibility test result.