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Simultaneous Determination of Danshensu and Protocatechuic Aldehyde in Compound Danshen Extract by HPLC

Chen Guang-bin

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Abstract

OBJECTIVE:To establish HPLC for simultaneous determination of Danshensu and protocatechuic aldehyde in compound Danshen extract.METHODS: The separation of samples was performed on an Agilent C18(250 mm×4.6 mm,5 μm) column with gradient elution for mobile phase which consisted of 1% acetic acid solution-methanol at a flow rate of 1 mL·min-1.The UV detective wavelength was set at 280 nm.RESULTS: Danshensu and protocatechuic aldehyde in compound Danshen extract were found to be free of interference from the foreign material.The calibration curves all were linear(r0.999) within their respectively range.The method recovery,reproducibility and stability of the samples were all up to the standard.CONCLUSION: The method is rapid and accurate and it can be used to control the internal quality of compound Danshen.

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What this paper is about

OBJECTIVE:To establish HPLC for simultaneous determination of Danshensu and protocatechuic aldehyde in compound Danshen extract.METHODS: The separation of samples was performed on an Agilent C18(250 mm×4.6 mm,5 μm) column with gradient elution for mobile phase which consisted of 1% acetic acid solution-methanol at a flow rate of 1 mL·min-1.The UV detective wavelength was set at 280 nm.RESULTS: Danshensu and protocatechuic aldehyde in compound Danshen extract were found to be free of interference from the foreign material.The calibration curves all were linear(r0.999) within their respectively range.The method recovery,reproducibility and stability of the samples were all up to the standard.CONCLUSION: The method is rapid and accurate and it can be used to control the internal quality of compound Danshen.

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Available abstract

OBJECTIVE:To establish HPLC for simultaneous determination of Danshensu and protocatechuic aldehyde in compound Danshen extract.METHODS: The separation of samples was performed on an Agilent C18(250 mm×4.6 mm,5 μm) column with gradient elution for mobile phase which consisted of 1% acetic acid solution-methanol at a flow rate of 1 mL·min-1.The UV detective wavelength was set at 280 nm.RESULTS: Danshensu and protocatechuic aldehyde in compound Danshen extract were found to be free of interference from the foreign material.The calibration curves all were linear(r0.999) within their respectively range.The method recovery,reproducibility and stability of the samples were all up to the standard.CONCLUSION: The method is rapid and accurate and it can be used to control the internal quality of compound Danshen.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Gradient elution, Protocatechuic acid, Acetic acid, Reproducibility, Methanol

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Simultaneous Determination of Danshensu and Protocatechuic Aldehyde in Compound Danshen Extract by HPLC — Research Paper | ScholarLens