Analysis of Danshensu and Protocatechuic Aldehyde in Compound Danshen Dropping Pill by High Performance Liquid Chromatoraphy

Objective To separate and determine Dunshensu and protocatechuic aldehyde in Compound Danshen Dropping Pill.Methods The chromatographic conditions were as follow:Nucleosil C18 column.A mixture of methanol-water-acetic acid glacial(19∶80∶1)as the mobile phase with a flow rate of 1 ml per minute and the detection wavelength at 279 nm.Phydroxybenoic acid was used as internal standard.Results The calibration curves were linear in the range of 10~80 mg/L for Danshensu and 2~16 mg/L for protocatechuic aldehyde.The average recovery of Danshensu and protocatechuic aldehyde were 99.4%and 99.5%,and RSD were 1.6%and 1.5%.Conclusion This method is accurate,simple and convenient,rapid and reproducible and can be used for the quality control of compound Danshen Dropping Pill.