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2011•China Animal Husbandry & Veterinary MedicineRequires access

Preparation of Monoclonal Antibody of PRRSV(CH-IR Strain)

Xin Zheng

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Abstract

The Balb/c mouse were immunized for several times with the purified PRRSV,the mouse spleen cells immunized were fused with SP2/0 cells.By indirect enzyme linked immunity assay and four 4 sub-cloning,we got one monoclonal antibody hybridoma cells line(3D6),it was the sub-type of IgG2a by identification,and the chromosome number was 99.The cells supernatant titer and ascites were 1∶512 and 1∶20480.This McAb did not occurred cross reaction with PRV,PPV and HCV.and showed excellent specificity.It could secret antibody stably for 20 generation.The McAb could be used to detect PRRSV antigen and would be useful for studying diagnostic methods of PRRSV.

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What this paper is about

The Balb/c mouse were immunized for several times with the purified PRRSV,the mouse spleen cells immunized were fused with SP2/0 cells.By indirect enzyme linked immunity assay and four 4 sub-cloning,we got one monoclonal antibody hybridoma cells line(3D6),it was the sub-type of IgG2a by identification,and the chromosome number was 99.The cells supernatant titer and ascites were 1∶512 and 1∶20480.This McAb did not occurred cross reaction with PRV,PPV and HCV.and showed excellent specificity.It could secret antibody stably for 20 generation.The McAb could be used to detect PRRSV antigen and would be useful for studying diagnostic methods of PRRSV.

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Available abstract

The Balb/c mouse were immunized for several times with the purified PRRSV,the mouse spleen cells immunized were fused with SP2/0 cells.By indirect enzyme linked immunity assay and four 4 sub-cloning,we got one monoclonal antibody hybridoma cells line(3D6),it was the sub-type of IgG2a by identification,and the chromosome number was 99.The cells supernatant titer and ascites were 1∶512 and 1∶20480.This McAb did not occurred cross reaction with PRV,PPV and HCV.and showed excellent specificity.It could secret antibody stably for 20 generation.The McAb could be used to detect PRRSV antigen and would be useful for studying diagnostic methods of PRRSV.

Key concepts: Monoclonal antibody, Spleen, Virology, Titer, Molecular biology, Biology, Antibody, Cloning (programming)

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