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2007•Progress in Veterinary MedicineRequires access

Preparation and Identification of Monoclonal Antibodies Against GP5 Protein PRRSV

Huanchun Chen

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Abstract

Balb/c mice were immunized with GST-GP5 fusion protein of PRRSV expressed by E.coli.A hybridoma cell line named 52-c which was able to steadily secrete monoclonal antibod(McAb) against GP5 protein of PRRSV was obtained after the amalgamation of mouse splenic cell with SP2/0 cell.The number of the chromosome in 52-C cell was 90 in average(85~96).Indirect ELISA analyses indicated that the immunoglobulin subtype of 52-C was IgG2a.The ELISA titer of the ascites induced by the hybridoma was 100×27.And the monoclonal antibody 52-C was specific to PRRSV,but it was not able to neutralize PRRSV.Indirect ELISA and Western blot indicated 52-C recognized endocellular epitope of GP5 protein.

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What this paper is about

Balb/c mice were immunized with GST-GP5 fusion protein of PRRSV expressed by E.coli.A hybridoma cell line named 52-c which was able to steadily secrete monoclonal antibod(McAb) against GP5 protein of PRRSV was obtained after the amalgamation of mouse splenic cell with SP2/0 cell.The number of the chromosome in 52-C cell was 90 in average(85~96).Indirect ELISA analyses indicated that the immunoglobulin subtype of 52-C was IgG2a.The ELISA titer of the ascites induced by the hybridoma was 100×27.And the monoclonal antibody 52-C was specific to PRRSV,but it was not able to neutralize PRRSV.Indirect ELISA and Western blot indicated 52-C recognized endocellular epitope of GP5 protein.

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Available abstract

Balb/c mice were immunized with GST-GP5 fusion protein of PRRSV expressed by E.coli.A hybridoma cell line named 52-c which was able to steadily secrete monoclonal antibod(McAb) against GP5 protein of PRRSV was obtained after the amalgamation of mouse splenic cell with SP2/0 cell.The number of the chromosome in 52-C cell was 90 in average(85~96).Indirect ELISA analyses indicated that the immunoglobulin subtype of 52-C was IgG2a.The ELISA titer of the ascites induced by the hybridoma was 100×27.And the monoclonal antibody 52-C was specific to PRRSV,but it was not able to neutralize PRRSV.Indirect ELISA and Western blot indicated 52-C recognized endocellular epitope of GP5 protein.

Key concepts: Monoclonal antibody, Epitope, Molecular biology, Antibody, Titer, Biology, Virology, Western blot

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