Preparation of monoclonal antibody of PRRSV(Resp strain)

An indirect enzyme linked immunosorbent assay was developed with purified PRRSV (Resp strain) and prepared from Marc145 cell line to detect monoclonal antibodies (McAbs) secreted by hybridoma cell lines. The BALB/C mouse were immunized for several times with the virus replicating on porcine alveolar macrophage, then the spleen cells of the hyper immunized mouse and SP 2/10 cells were fused with PEG 1500. The results showed that the cell fusion rate was 70 6%,positive rate was 6 3%, and two hybridoma cell lines secreting monoclonal antibodies were obtained after sub cloning. The McAbs could be used to detect PRRSV antigen in cells with IFA. It would be useful for studying the immunity of PRRSV isolates and improving present diagnostic methods.