Influence of Advanced Glycation End Products on the Function of Schwann Cells in Vitro and Related Mechanism

Objective To study the influence of advanced glycation end products(AGEs) on rat sciatic nerve-derived Schwann cells in vitro and investigate the change of inflammatory factors in order to provide a novel therapeutic target of peripheral neuropathy in diabetes patients.Methods Primary sciatic nerve-derived Schwann cells from 3-day neonatal SD rats were obtained,purified and then identified by immunocytochemistry staining.The Schwann cells were divided into seven groups and were treated respectively with pure culture medium(control group),20 μg/ml BSA,20 μg/ml AGEs,50 μg/ml BSA,50 μg/ml AGEs,100 μg/ml BSA and 100 μg/ml AGEs respectively.Then the proliferation,migration,apoptosis and Caspase-3 mRNA of treated cells were detected.After that,the Schwann cells were divided into four groups and then were treated with pure culture medium(control group),50 μg/ml BSA,50 μg/ml AGEs and 50 μg/ml AGEs+10 μg/ml anti-receptor of AGEs(RAGE) antibody respectively.The activity of NF-κB and the level of tumor necrosis factor α(TNF-α) and interleukin 6(IL-6) were measured.Results(1)Compared with the control group,there were no statistically significant difference on every detective parameter(proliferation,migration,apoptosis and Caspase-3 mRNA) in BSA groups(P0.05).The cell proliferation,migration in AGEs groups became significantly weaker than those in BSA groups and control group(P0.05);The cell apoptosis rate and Capases-3 mRNA in AGEs groups were significantly higher than those in BSA groups and control group(P0.05).There were significant differences among the three AGEs groups(P0.05).(2) Additionally,the NF-κB,TNF-α and IL-6 in 50 μg/ml BSA group showed no statistically significant difference compared with the control group,but the above indexes in the 50 μg/ml AGEs group were significantly increased compared to BSA group and the control group(P0.05).However,there was no significant difference between BSA group and AGEs+anti-RAGE antibody group(P0.05).Conclusion AGEs can inhibit the proliferation capacity of Schwann cells and accelerate the apoptosis in vitro,probably through promoting the production of inflammatory factors which are harmful to cells.