Effects of Advanced Glycosylation End Products on Proliferation of Human Colon Carcinoma Cell Line SW-480 and Its Mechanism

Objective To observe the effects of advanced glycosylation end products(AGE) on proliferation of SW-480 cells and study the possible mechanism.Methods Various concentrations of AGE were designed to have impact on SW-480 cells.Proliferation of SW-480 cells was assessed by thiazolyl blue tetrazolium bromide(MTT) assay;The impact of AGE on the cell cycle of SW-480 cells was analyzed by flow cytometry(FCM);the influence of AGE on expression of CyclinD1 was checked by Western blotting;and the impact of AGE on telomerase activity was examined by telomeric repeat amplification proctol(TRAP) sliver staining.For the MTT assay,blank control group,100 μg/mL bovine serum albumin(BSA) group,50,100 and 500 μg/mL AGE groups were designed,while for other examinations,there were only 100 μg/mL BSA group and 100 μg/mL AGE group.Results MTT result showed that AGE increased the proliferation of SW-480 cells in a dose-dependent mode.The proportion of the cells at G0/G1 stage of the 100 μg/mL BSA group and the 100 μg/mL AGE experimental group were(56.02±0.58)% and(51.93±1.01)% respectively after 72 hours,with a significant difference(P0.05);western blotting showed that the expression of CyclinD1 in the 100 μg/mL AGE group was significantly higher than that in the 100 μg/mL BSA group after 72 hours;TRAP silver staining demonstrated that telomerase activity increased significantly after treated with 100 μg/mL AGE for 72 hours.Conclusions AGE can promote the growth of SW-480 cells in a dose-dependent mode.Its mechanism is mainly by up-regulating the expression of CyclinD1 to shorten G0/G1 and increasing the telomerase activity significantly.