Effect of Tanshinone IIA on RAGE Expression and Oxidative Stress Status in Human Mesangial Cells Induced by AGE

Objective To investigate the effect of Tanshinone ⅡA( TanⅡA) on the expression of receptor for advanced glycated endproducts( RAGE) and the oxidative stress status of human mesangial cells induced by AGE. Methods Human mesangial cells( HMCs) were cultured with various concentration of AGE- BSA( 1. 0 μg /ml,10. 0 μg /ml,50. 0 μg /ml and 100. 0 μg /ml),and then were treated with Tan ⅡA( 0. 1 μg /ml,1. 0 μg /ml,5. 0 μg /ml and 10. 0 μg /ml). The cultured HMCs were divided into AGE group,AGE + TanⅡ A group and control group. By using GAPDH as reference,the protein and mRNA expression of RAGE were evaluated by Western blotting analysis and real- time PCR respectively. The activity of superoxide dismutase( SOD),glutathione peroxidase( GSH- Px) and malonaldehyde( MDA) levels in the supernatant of HMCs were measured. Results The expressions of RAGE and mRNA in HMCs between control group and different concentration of AGE groups all showed statistically significant differences( F = 4. 428 and 5. 031,P 0. 05). Compared with the control group,the expressions of RAGE and mRNA in HMCs of 10. 0 μg /ml,50. 0 μg /ml and 100. 0 μg /ml AGE groups were significantly higher( P 0. 05). The expressions of RAGE and mRNA in HMCs between control group and AGE +different concentrations of TanⅡ A groups all showed statistically significant differences( F = 5. 002 and 5. 312,P 0. 05). Compared with AGE + 0 μg /ml TanⅡA group,the expressions of RAGE and mRNA in HMCs of the control group,AGE +0. 1 μg/ml TanⅡ A group,AGE + 1. 0μg/ml TanⅡ A group,AGE +5. 0 μg/ml TanⅡ A group and AGE + 10. 0 μg/ml TanⅡ A group were significantly lower( P 0. 05). The SOD,GSH- Px and MDA levels in the supernantant of HMCs showed statistically significant differences between the control group,different concentrations of AGE group and AGE + TanⅡ A group( P 0. 05). Conclusion TanⅡ A could significantly reduce the RAGE and mRNA expression in HMCs induced by AGE,and the oxidative stress levels are also improved.