Expression and significance of Skp2,PTEN and p27 in human glioma

Objective To investigate the significance and the expression of Skp2,PTEN and p27 in the human glioma,and their relationships.Methods The expression of Skp2,PTEN and p27 in 33 cases of human gliomas and 6 specimens of the normal brain tissue was detected by immunohistochemical technique.Results In normal brain tissue,groupsⅠ,ⅡandⅢ,Ⅳ,the positive expression level of Skp2 was 3.33±1.21,27.11±9.29 and 59.53±17.84 respectively,that of PTEN was 89.33±6.89, 57.67±14.47 and 8.13±3.96 respectively,and that of p27 was 79.83±10.11,60.83±14.04 and 13.07±5.66 respectively.There were significant differences in the expression levels of Skp2,PTEN and p27 among the normal brain tissue,the gliomas of gradesⅠ,Ⅱand the gliomas of gradesⅢ,Ⅳ(P＜0.01).Inverse correlation was found between protein of Skp2 and p27 in glioma,also between Skp2 and PTEN.There was a positive correlation between PTEN and p27.Conclusion The expression of Skp2 was increased due to the mutation of PTEN in gliomas.Therefore the degradation of p27 in the ubiquitin- proteasome system was promoted.Skp2 may play an important role in the development of glioma by its promotive effect on decomposition of p27 and it may function as a critical component in the pathway of PTEN regulating p27 and cell proliferation in gliomas.