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Making lung cancer related cDNA microarray

Fan Bao

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Abstract

This study aimed at introducing a method of making lung cancer related cDNA Microarray and its using in gene expression profile. 60 lung cancer related genes were cloned, purified and micro?arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from passage 0, 20, 35 in malignant transformation cell model was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA. After scanning using ScanArray 3000, It showed analysised pots with uniformity background and clear signal.

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What this paper is about

This study aimed at introducing a method of making lung cancer related cDNA Microarray and its using in gene expression profile. 60 lung cancer related genes were cloned, purified and micro?arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from passage 0, 20, 35 in malignant transformation cell model was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA. After scanning using ScanArray 3000, It showed analysised pots with uniformity background and clear signal.

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Available abstract

This study aimed at introducing a method of making lung cancer related cDNA Microarray and its using in gene expression profile. 60 lung cancer related genes were cloned, purified and micro?arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from passage 0, 20, 35 in malignant transformation cell model was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA. After scanning using ScanArray 3000, It showed analysised pots with uniformity background and clear signal.

Key concepts: Complementary DNA, Microarray, Lung cancer, Biology, Molecular biology, Cancer, Microarray analysis techniques, Gene

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