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Test the Expression of Liver cDNA by Microarray

Hongju Mao

Abstract

 Objective To investigate the experession profile of liver cDNA clones． Methods By means of hybridization of genes on microarray slide with normal liver mRNA and by comparing hybridization signals on slide genes the differential expression genes were examined． Results 1487 of 1500 cDNA clones on slides expressed in normal liver and 97expressed in higher level 1010 expressed in middle level 380 expressed in lower level． Conclusion cDNA microarray quantitative analysis is an effective method to test expression defference．

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Key concepts: Complementary DNA, Microarray, Biology, Gene, Gene expression, Microarray analysis techniques, Molecular biology, Genetics