2003•Unpublished venueRequires access
Identification of differentially expressed genes in different malignant transformation phases of BEP2D cell line
Baoxing Fan, Kaitai Zhang, Jing-Fen Sun, Xiang Xiaoqiong, Ling Xie, Yingchun Hu, Shengqi Wang, Wu Dechang
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Abstract
Objective To screen and identify differentially expressed genes in different malignant transformation phased of BEP2D cell line induced by alpha-particles. Methods Suppression subtractive hybridization(SSH) and cDNA microarray were performed. Results Three suppression subtractive cDNA libraries were constructed.BEP2D cDNA library contained 416 clones,R15H20 cDNA library contained 301 clones,and R15H35 cDNA library contained 586 clones.After confirmed by cDNA microarray,the positive rates in BEP2D cDNA microarray were 90.4%,21.6% and 19.7%;in R15H20 cDNA microarray,8.6%,93.8% and 31.6%;and in R15H35 cDNA microarray,23.5%,18.2%,90.7% when hybridized with probes coming from BEP2D,R15H20 and R15H35 cells,respectively. Conclusions The combination of SSH and cDNA microarray is a rapid and effective method for high throughout screening and identification of differentially expressed genes in different samples.The cDNAs confirmed by cDNA microarray may be the relative genes that associate with malignant transformation of bronchial epithelial cells induced by alpha-particles.