2011•Unpublished venueRequires access
Effect of different glucose concentrations on proliferation and apoptosis of pancreatic islet β-cells in mice
Li-Chao Ma
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Abstract
Objective To study the effect of different glucose concentrations on proliferation and apoptosis of pancreatic islet β-cells in mice.Methods Proliferation and apoptosis of MIN-6 cells were detected by MTT assay and cytometry,respectively with Annexin V-FITC staining after they were cultured with glucose at different concentrations(5.6,11.1,16.6,25.0 and 33.3mmol/L)for 72h.Results The proliferation rate of MIN-6 cells was significantly lower while the apoptosis rate of MIN-6 cells in mice was significantly higher 72h after they were cultured with glucose at the concentrations of 5.6,11.1 and 33.3mmol/L than at the concentration of 25mmol/L(P0.05).Conclusion The proliferation of MIN-6 cells can be inhibited while the apoptosis of MIN-6 cells can be increased in mice by glucose at the concentrations of25mmol/L and25mmol/L.