The Effect of Dexamethasone on Cell Proliferation and Apoptosis in Murine MIN6 Pancreatic β-cells

Objective To investigate the effect of dexamethasone on cell proliferation and apoptosis in murine MIN6 pancreatic β-cells.Methods To establish the apoptosis model by choosing the murine MIN6 pancreatic β-cells as the objects of the study,which were cultured in vitro and induced by dexamethasone with the concentrations ranging from 50 to 800 nmol/L for 1～4 d.MTT assay was adoped to measure the cell survival rate after treatment with dexamethasone,the apoptotic rate was observed through fluorsecence microsopic analysis with hochest/PI staining.Results The results from the 4 times MTT assays reveal that after exposure to dexamethasone with the concentrations ranging from 50 to 800 nmol/L for 1～4 d,the growth of MIN6 cells was significantly inhibited.Hochest/PI staining proved that when treated by dexamethasone with the concentration over 100 nmol/L for 48 hours,the cells' apoptotic rate was significant,reaching up to(30.44±4.52)%,(P0.01 compared with control group).The cells were induced from apoptosis to necrosis with 800 nmol/L dexmethasone.Conclusion The dexmethasone has an evident effect on the proliferation of the murine MIN6 pancreatic β-cells,and could induce apoptosis.