The Influence of 2-deoxy-D-glucose on Apoptosis of Human Ovarian Cancer Cell Line SKOV-3

Objective To investigate the influence of 2-deoxy-D-glucose(2-DG) on proliferation and apoptosis of human ovarian cancer SKOV-3 cells.Methods The human ovarian cancer SKOV-3 cells were treated with 2-DG in different concentrations(2.5 mmol/L 、5 mmol/L、10 mmol/L、20 mmol/L、40 mmol/L).Then MTT was used to detect the cell growth inhibition rate of each group at different time;Hoechst33258 staining assay was chosen to observe the morphological changes induced by 2-DG;the cell cycle and apoptosis were analyzed via flow cytometry.Results Different concentration of 2-DG can inhibit the proliferation of SKOV-3 cells in a dose and time-dependent manner,and there was significant difference compared with control group(P0.05);Our results also showed 2-DG treated cells were arrested in G0/G1 phase;Apoptosis assay by Flow showed admonsted that 2-DG can induce cell apoptosis or necrosis.Conclusion 2-DG has inhibitory effect on the proliferation of SKOV-3 cells in vitro,and the effect is dose and time-dependent.