Screening of RAPD Molecular Marker Related to Gynoecious Gene in Cucumber(Cucumis sativus L.)

The genomic DNA is extracted from cucumber with the method of CTAB.RAPD technique is employed to study the molecular markers related to gynoecious gene of cucumber.In this paper,39 randomly selected 10 bp primers is screened,of which 12 primers show polymorphism in cucumber tested.These polymorphic primers are further screened by PCR.The results show that a specific band of about 2000 bp,scored as S10-2 kb,can be amplified stably and clearly in cucumber of gynoecious lines with the primer of S10,while the band is not found in monoecious cucumber.A full length of 2003 bp is obtained by sequencing the cloned fragment.Then the sequence is analyzed by Blast on NCBI. Sequence analysis shows that the S10-2 kb marker is a new one harboring a largest ORF coding a short peptide of 62-amino-acid residues.The function of this peptide is not determined by now.