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2005•Journal of Hangzhou Teachers CollegeRequires access

Effective extraction of genomic DNA and RAPD analysis on different sex types of cucumber

YU Ai-hua

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Abstract

The genomic DNA was effectively extracted from 11 different sex types of cucumber(Cucumis sativus L.)via developed isopropanol-precipitating method. DNA was employed as template for RAPD analysis. A total of 22 arbitrary 10-mer oligonucleotide primers were screened using RAPD technique on the genomic DNA. Among them, two primers of S23 and S91 can produced repeatable polymorphic markers between gynoecious cultivar MT700 and the others. The further studies on the polymorphic markers from S23 and S91 are expected.

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What this paper is about

The genomic DNA was effectively extracted from 11 different sex types of cucumber(Cucumis sativus L.)via developed isopropanol-precipitating method. DNA was employed as template for RAPD analysis. A total of 22 arbitrary 10-mer oligonucleotide primers were screened using RAPD technique on the genomic DNA. Among them, two primers of S23 and S91 can produced repeatable polymorphic markers between gynoecious cultivar MT700 and the others. The further studies on the polymorphic markers from S23 and S91 are expected.

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Available abstract

The genomic DNA was effectively extracted from 11 different sex types of cucumber(Cucumis sativus L.)via developed isopropanol-precipitating method. DNA was employed as template for RAPD analysis. A total of 22 arbitrary 10-mer oligonucleotide primers were screened using RAPD technique on the genomic DNA. Among them, two primers of S23 and S91 can produced repeatable polymorphic markers between gynoecious cultivar MT700 and the others. The further studies on the polymorphic markers from S23 and S91 are expected.

Key concepts: RAPD, genomic DNA, Cucumis, Biology, Plant reproductive morphology, DNA, Genetics, DNA extraction

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