Construction of the High-efficiency Regeneration System of Blueberry with Its Leaves

Vaccinium corymbosum leaves were used as the explants to study quiet a few factors affecting in vitro regeneration and construct its high-efficiency regeneration system.The results indicated that the modified 1/2 MS with 5 mg·L~(-1) Zeatin(t-ZR) was the optimal medium for adventitious bud differentiation and its regeneration rate reached as high as 100%.The leaves at different nods showed significant effects on adventitious-bud differentiation of blueberry leaves and the leaves on the first~second nodes were liable to differentiate adventitious buds;Medium contacts of distal leaf surface,artificial leaf wounds,and nil-darkness or short-time dark treatments were conductive to adventitious bud differentiation.IBA at low concentration are favorable for leaf-regenerated stems to root and on the modified 1/2 MS with 0.1 mg·L~(-1) IBA,the rooting rate of bluecrop was 66.7% whereas that of berkly was 33.3%.