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2006•Shandong Nongye Daxue xuebaoRequires access

BUD REGENERATION SYSTEM FROM LEAVES OF POPULUS×DELTOSINONIPYRAMIDALIS

Jihong Li

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Abstract

Adventitious bud regeneration from the leaves of Populus ×Deltosinonipyramidalis cv.Zhaiguanheiqingyang 31,6(Lu31,Lu6)in vitro with different media and hormones of tissue culture was studied.The results showed that the optimum medium for adventitious bud regeneration was 1/2MS+BA0.3mg/L+NAA0.01～0.05mg/L, and the frequency of adventitious bud regeneration could exceed 90.0%,the number of adventitious bud got to 10 to 13/ the best leaves for adventitious bud regeneration from the leaves which were cultured on medium are the top lstto 3rd ones.

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What this paper is about

Adventitious bud regeneration from the leaves of Populus ×Deltosinonipyramidalis cv.Zhaiguanheiqingyang 31,6(Lu31,Lu6)in vitro with different media and hormones of tissue culture was studied.The results showed that the optimum medium for adventitious bud regeneration was 1/2MS+BA0.3mg/L+NAA0.01～0.05mg/L, and the frequency of adventitious bud regeneration could exceed 90.0%,the number of adventitious bud got to 10 to 13/ the best leaves for adventitious bud regeneration from the leaves which were cultured on medium are the top lstto 3rd ones.

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Available abstract

Adventitious bud regeneration from the leaves of Populus ×Deltosinonipyramidalis cv.Zhaiguanheiqingyang 31,6(Lu31,Lu6)in vitro with different media and hormones of tissue culture was studied.The results showed that the optimum medium for adventitious bud regeneration was 1/2MS+BA0.3mg/L+NAA0.01～0.05mg/L, and the frequency of adventitious bud regeneration could exceed 90.0%,the number of adventitious bud got to 10 to 13/ the best leaves for adventitious bud regeneration from the leaves which were cultured on medium are the top lstto 3rd ones.

Key concepts: Regeneration (biology), Biology, Botany, Tissue culture, In vitro, Cell biology, Biochemistry

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